A lab experiment was conducted during summer session of academic year 2014-15 at Department of Biochemistry and Molecular biology, MGM College of Agricultural Biotechnology, Gandheli, Aurangabad to synthesize and characterize silver nanoparticles from different plant extracts and to check the antibacterial effects on S. mutans .The experiment was laid out in Completely Randomized Design (CRD) with 13 treatments for synthesis of silver nanoparticles. Silver nanoparticles were confirmed by color change, UV-VIS spectrometer, FTIR test and SEM. We found 13 positive samples which were used for antibacterial assay against S. mutans.
All the concentrations of the silver nanoparticles which were applied for antibacterial assay showed significantly superior results over the control. Moreover, these nanoparticles showed considerable antimicrobial activity to Bacterial strains i.e. S. mutans. Vad silver nanoparticles showed highest zone of inhibition for S. mutans i.e. 18.33 mm.
In conclusion, the present investigation demonstrated that extracts obtained from the leaf of different plants were capable of producing silver nanoparticles from an aqueous solution of AgNO3. The method described a novel application of leaf extract of 13 plants and fortifies the screening of a new plant as a potential source of reducing and capping agent for the synthesis of silver nanoparticles. The synthesized silver nanoparticles were well capped and showed a strong antibacterial activity which is very important from the aspects of its bio-medical application.
